Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsy specimens.

Compression of Histopathology, Culture and Rapid Urease Test in Diagnosis of Helicobacter Pylori in Gastric Biopsy Specimens

Abstract Background and Objective: Helicobacter pylori (H. Pylori) infection is related to chronic gastritis, peptic ulcer, duodenal ulcer and gastric cancer. Thus, identification and treatment of the infection have a considerable importance. The aim of this study was to compare three methods of Histopathology, Culture and Rapid Urease test (RUT) in identification of H. Pylori in gastric bio...

Resistance in Gastric Biopsy Specimens Helicobacter pylori Clarithromycin Nucleic Acid Probes for Detection of Hybridization Method Using Peptide Validation of a Fluorescence In Situ

Published Ahead of Print 17 April 2013. 10.1128/JCM.00302-13. 2013, 51(6):1887. DOI: J. Clin. Microbiol. Charles W. Keevil, Maria J. Vieira and Nuno F. Azevedo Pimentel-Nunes, Mário Dinis-Ribeiro, Céu Figueiredo, Mónica Oleastro, Fátima Carneiro, Catarina Brandão, Pedro Laura Cerqueira, Ricardo M. Fernandes, Rui M. Ferreira, Resistance in Gastric Biopsy Specimens Helicobacter pylori Clarithromy...

PCR and RFLP Analysis for Identification and Typing of Helicobacter pylori Strains from Gastric Biopsy Specimens

Validation of a fluorescence in situ hybridization method using peptide nucleic acid probes for detection of Helicobacter pylori clarithromycin resistance in gastric biopsy specimens.

Here, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection in paraffin-embedded gastric biopsy specimens. Both a retrospective study and a prospective cohort study were conducted to evaluate the specificity and sensitivity of a PNA-FISH method to determ...

TLR4 and TLR2 Expression in Biopsy Specimens from Antral and Corporal Stomach Zones in Helicobacter pylori Infections

Background: It is not yet known which types of Toll-like receptors (TLRs) are most effective in Helicobacter pylori (H. pylori) recognition. It is also not known which gastric zones have the most prominent roles in TLR-mediated bacterial recognition. The aim of this work was to analyze the expression of TLR2 and TLR4 in biopsy specimens from H. pylori-infected patients. Methods: Thirty-eight...